1、 Overview

Tuberculosis- γ The principle of interferon release test (TB-IGRA) is as follows:

The specific stimulating antigens "esat6 and cfp10" are unique fragments of pathogenic Mycobacterium tuberculosis but not of BCG and other mycobacteria.

Mycobacterium tuberculosis infected persons have specific T lymphocytes for each antigen, which can recognize and present these antigens, stimulate the proliferation of T lymphocytes, and release cytokines, such as IFN- γ。 Specific IFN released in vitro can be quantitatively detected by ELISA- γ， So as to judge whether it is infected with Mycobacterium tuberculosis.

2、TB-IGRAprocess

A、Schematic diagram of stimulation process:

B、Schematic diagram of detection process:

3、 Recommended usage（ELISA）

A、Amount of stimulator: 1ug/tube

B、Recommendations for use of ELISA reagents:

C6H4：1:1000Coated with antibody overnight. Washing and sealing. Sampling100ul，Joining at the same time20ul 1:2000Diluted8A11-F(ab)2-HRP，Washing, color development and termination.

C、Quantitative range of standard

Take the mean absorbance of the calibration sample as the independent variable（X），Its correspondingIFN-γContent of（12.5 pg/ml~400 pg/ml）Is a dependent variable（Y），The linear regression equation is obtained by power fittingy=167.64x^0.9554，The data and schematic diagram are as follows：

4、 Result judgment recommendation

Test culture tube (T) content value=T, background control culture tube (N) content value=N, positive control culture tube (P) content value=P (unit: pg/ml)

5、 Performance evaluation

*Refer to Australian QFT reagent

6、 Raw material information